{"id":87289,"date":"2025-09-09T00:11:03","date_gmt":"2025-09-08T22:11:03","guid":{"rendered":"https:\/\/www.infokuryr.cz\/n\/?p=87289"},"modified":"2025-09-08T12:33:38","modified_gmt":"2025-09-08T10:33:38","slug":"nova-studie-zbytky-dna-a-sekvence-sv40-v-mrna-vakcin-proti-covid-19","status":"publish","type":"post","link":"https:\/\/www.infokuryr.cz\/n\/2025\/09\/09\/nova-studie-zbytky-dna-a-sekvence-sv40-v-mrna-vakcin-proti-covid-19\/","title":{"rendered":"Nov\u00e1 studie: Zbytky DNA a sekvence SV40 v mRNA vakc\u00edn proti COVID-19"},"content":{"rendered":"
<\/div>\n

Studie publikovan\u00e1 6. z\u00e1\u0159\u00ed 2025 v \u010dasopise\u00a0<\/span>Autoimmunity<\/span><\/a><\/em>\u00a0vyvol\u00e1v\u00e1 kontroverze: T\u00fdm veden\u00fd Davidem J. Speicherem spolu s Jessicou Rose a Kevinem McKernan (Medicinal Genomics) zkoumal mRNA vakc\u00edny od spole\u010dnost\u00ed Pfizer-BioNTech a Moderna. Nalezli m\u011b\u0159iteln\u00e9 mno\u017estv\u00ed zbytkov\u00e9 plazmidov\u00e9 DNA \u2013 a v lahvi\u010dk\u00e1ch Pfizer i dal\u0161\u00ed fragment obsahuj\u00edc\u00ed tzv.\u00a0<\/span>sekvence promotoru a zesilova\u010de SV40<\/span><\/strong>\u00a0.<\/span><\/p>\n

Kl\u00ed\u010dov\u00e1 ot\u00e1zka: P\u0159ekra\u010duj\u00ed tyto zbytky DNA mezin\u00e1rodn\u011b platn\u00e9 limity \u2013 a pokud ano, jak\u00fd to m\u00e1 v\u00fdznam pro bezpe\u010dnost a schv\u00e1len\u00ed?<\/span><\/p>\n

N\u00e1vrh a metody studie<\/span><\/h3>\n
    \n
  • Vzorky:<\/span><\/strong>\u00a032 lahvi\u010dek ze 16 \u0161ar\u017e\u00ed (10 Pfizer \/ 22 Moderna) z kanadsk\u00fdch l\u00e9k\u00e1ren.<\/span><\/li>\n
  • Testy:<\/span><\/strong><\/li>\n<\/ul>\n
      \n
    • \n
        \n
      • qPCR pro spike sekvenci, plasmid ori a elementy SV40.<\/span><\/li>\n
      • Fluorometrie pro m\u011b\u0159en\u00ed celkov\u00e9ho mno\u017estv\u00ed nukleov\u00e9 kyseliny (v\u010detn\u011b DNA).<\/span><\/li>\n
      • Nanoporov\u00e9 sekvenov\u00e1n\u00ed lahvi\u010dky pro odhad d\u00e9lky fragmentu.<\/span><\/li>\n
      • Test DN\u00e1zy I k ur\u010den\u00ed, zda byla DNA chr\u00e1n\u011bna v lipidov\u00fdch nano\u010d\u00e1stic\u00edch (LNP).<\/span><\/li>\n<\/ul>\n<\/li>\n
      • Dopln\u011bk:<\/span><\/strong>\u00a0Explorativn\u00ed korelace hladin DNA s hl\u00e1\u0161en\u00edmi ne\u017e\u00e1douc\u00edch \u00fa\u010dink\u016f VAERS.<\/span><\/li>\n<\/ul>\n

        Kl\u00ed\u010dov\u00e9 v\u00fdsledky<\/span><\/h3>\n
          \n
        • qPCR:<\/span><\/strong>\u00a0V\u0161echny lahvi\u010dky Pfizer byly pozitivn\u00ed na Spike, Ori a SV40; lahvi\u010dky Moderna byly pozitivn\u00ed na Spike\/Ori, ale negativn\u00ed na SV40.<\/span><\/li>\n
        • Mno\u017estv\u00ed:<\/span><\/strong>\u00a0Pfizer: 0,22\u20137,28 ng DNA\/d\u00e1vka; Moderna: 0,01\u20130,78 ng. Ve dvou \u0161ar\u017e\u00edch p\u0159\u00edpravku Pfizer p\u0159ekro\u010dily hladiny SV40 limit 10 ng stanoven\u00fd WHO\/FDA.<\/span><\/li>\n
        • Fluorometrie:<\/span><\/strong>\u00a0V\u00fdrazn\u011b vy\u0161\u0161\u00ed hodnoty \u2013 Pfizer: 371\u20131 548 ng\/d\u00e1vka, Moderna: 1 130\u20136 280 ng\/d\u00e1vka. Tyto hodnoty jsou 36\u2013627kr\u00e1t vy\u0161\u0161\u00ed ne\u017e doporu\u010den\u00ed WHO\/FDA.<\/span><\/li>\n
        • Nanop\u00f3r:<\/span><\/strong>\u00a0Fragmenty o pr\u016fm\u011brn\u00e9 d\u00e9lce 214 bp a\u017e 3,5 kb.<\/span><\/li>\n
        • DN\u00e1za I:<\/span><\/strong>\u00a0DNA z\u016fstala p\u0159ev\u00e1\u017en\u011b stabiln\u00ed \u2013 co\u017e nazna\u010duje ochranu LNP.<\/span><\/li>\n
        • Extrapolace:<\/span><\/strong>\u00a0Miliardy fragment\u016f DNA na d\u00e1vku, pravd\u011bpodobn\u011b propustn\u00e9 pro bu\u0148ky prost\u0159ednictv\u00edm zapouzd\u0159en\u00ed LNP.<\/span><\/li>\n<\/ul>\n

          Omezen\u00ed<\/span><\/h3>\n
            \n
          • P\u016fvod vzorku:<\/span><\/strong>\u00a0lahvi\u010dky z l\u00e9k\u00e1ren, \u017e\u00e1dn\u00fd v\u00fdb\u011br v\u00fdrobce, sekvenov\u00e1na pouze jedna lahvi\u010dka.<\/span><\/li>\n
          • Metodologie:<\/span><\/strong>\u00a0qPCR a fluorometrie nejsou validov\u00e1ny podle mezin\u00e1rodn\u00edch standard\u016f; mo\u017en\u00e9 zkreslen\u00ed m\u011b\u0159en\u00ed v d\u016fsledku zbytk\u016f RNA nebo LNP.<\/span><\/li>\n
          • Rozd\u00edly:<\/span><\/strong>\u00a0qPCR a fluorometrie poskytuj\u00ed velmi odli\u0161n\u00e9 v\u00fdsledky.<\/span><\/li>\n
          • Limity:<\/span><\/strong>\u00a0Hodnota WHO\/FDA (10 ng\/d\u00e1vka) poch\u00e1z\u00ed ze star\u0161\u00edch typ\u016f vakc\u00edn; jej\u00ed p\u0159enositelnost na mRNA LNP je nejasn\u00e1.<\/span><\/li>\n
          • Klinick\u00fd v\u00fdznam:<\/span><\/strong>\u00a0Nejsou k dispozici \u017e\u00e1dn\u00e9 \u00fadaje o zdravotn\u00edch v\u00fdsledc\u00edch. Srovn\u00e1n\u00ed VAERS jsou \u010dist\u011b pr\u016fzkumn\u00e1 a nejsou pr\u016fkazn\u00e1.<\/span><\/li>\n
          • St\u0159et z\u00e1jm\u016f:<\/span><\/strong>\u00a0Jeden z autor\u016f pracuje pro v\u00fdrobce pou\u017eit\u00fdch testovac\u00edch sad.<\/span><\/li>\n<\/ul>\n

            Z\u00e1v\u011br a v\u00fdznam<\/span><\/h3>\n

            Studie poukazuje na\u00a0<\/span>analyticky v\u011brohodn\u00fd sign\u00e1l<\/span><\/strong>\u00a0: zbytkovou DNA, n\u011bkdy ve vysok\u00e9m mno\u017estv\u00ed, a tak\u00e9 sekvence SV40 v lahvi\u010dk\u00e1ch Pfizeru. To neznamen\u00e1\u00a0<\/span>,<\/span><\/strong>\u00a0\u017ee jsou viry SV40 p\u0159\u00edtomny nebo \u017ee automaticky existuje zdravotn\u00ed riziko.<\/span><\/p>\n

            Zji\u0161t\u011bn\u00ed v\u0161ak vyvol\u00e1vaj\u00ed ot\u00e1zky pro\u00a0<\/span>v\u00fdrobce a regula\u010dn\u00ed org\u00e1ny<\/span><\/strong>\u00a0. Zejm\u00e9na kolik DNA (a jak\u00fd typ) je p\u0159ijateln\u00e9 ve vakc\u00edn\u00e1ch s mRNA LNP a zda je t\u0159eba upravit st\u00e1vaj\u00edc\u00ed limity.<\/span><\/p>\n

            Auto\u0159i po\u017eaduj\u00ed nez\u00e1visl\u00e9, standardizovan\u00e9 n\u00e1sledn\u00e9 studie s v\u011bt\u0161\u00edm vzorkem. Klinick\u00fd v\u00fdznam zji\u0161t\u011bn\u00ed z\u016fst\u00e1v\u00e1 nejasn\u00fd \u2013 je zapot\u0159eb\u00ed dal\u0161\u00edho v\u00fdzkumu a objasn\u011bn\u00ed.<\/span><\/p>\n

            \"\"<\/p>\n

            \"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

            Studie publikovan\u00e1 6. z\u00e1\u0159\u00ed 2025 v \u010dasopise\u00a0Autoimmunity\u00a0vyvol\u00e1v\u00e1 kontroverze: T\u00fdm veden\u00fd Davidem J. Speicherem spolu s…<\/p>\n","protected":false},"author":2,"featured_media":87290,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[7],"tags":[895,839,651],"_links":{"self":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/posts\/87289"}],"collection":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/comments?post=87289"}],"version-history":[{"count":0,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/posts\/87289\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/media\/87290"}],"wp:attachment":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/media?parent=87289"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/categories?post=87289"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/tags?post=87289"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}