{"id":113534,"date":"2026-08-19T00:12:46","date_gmt":"2026-08-18T22:12:46","guid":{"rendered":"https:\/\/www.infokuryr.cz\/n\/?p=113534"},"modified":"2026-08-18T12:26:59","modified_gmt":"2026-08-18T10:26:59","slug":"priprava-na-pandemii-z-laboratore-vakcinu-proti-h5n1-s-geneticky-modifikovanou-slozkou-moru-financuji-americti-danovi-poplatnici","status":"publish","type":"post","link":"https:\/\/www.infokuryr.cz\/n\/2026\/08\/19\/priprava-na-pandemii-z-laboratore-vakcinu-proti-h5n1-s-geneticky-modifikovanou-slozkou-moru-financuji-americti-danovi-poplatnici\/","title":{"rendered":"P\u0159\u00edprava na pandemii z laborato\u0159e: Vakc\u00ednu proti H5N1 s geneticky modifikovanou slo\u017ekou moru financuj\u00ed ameri\u010dt\u00ed da\u0148ov\u00ed poplatn\u00edci"},"content":{"rendered":"<div id=\"fb-root\"><\/div>\n<div class=\"elementor-element elementor-element-1fb4c2c elementor-widget elementor-widget-theme-post-content\" data-id=\"1fb4c2c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"theme-post-content.default\">\n<div class=\"elementor-widget-container\">\n<p class=\"wp-block-paragraph\"><strong><span dir=\"auto\">Ameri\u010dan\u00e9 za to plat\u00ed a mohli by b\u00fdt po\u017e\u00e1d\u00e1ni, aby to p\u0159evzali.<\/span><\/strong><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Ministerstvo zdravotnictv\u00ed a soci\u00e1ln\u00edch slu\u017eeb (HHS) financuje v\u00fdzkum experiment\u00e1ln\u00ed vakc\u00edny pro boj s budouc\u00ed pandemi\u00ed pta\u010d\u00ed ch\u0159ipky H5N1, kter\u00e1 kombinuje chim\u00e9rick\u00fd (\u201eFrankenstein\u016fv\u201c) protein pta\u010d\u00ed ch\u0159ipky H5 se syntetickou kopi\u00ed geneticky modifikovan\u00e9 lipidov\u00e9 slo\u017eky bakterie\u00a0<\/span><em><span dir=\"auto\">Yersinia pestis<\/span><\/em><span dir=\"auto\">\u00a0, kter\u00e1 zp\u016fsobuje mor.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Tento krok p\u0159ich\u00e1z\u00ed pot\u00e9, co prezident Donald Trump ned\u00e1vno obsadil dv\u011b nejvy\u0161\u0161\u00ed pozice ve vl\u00e1dn\u00edm apar\u00e1tu pro koordinaci pandemie: Sara Brennerov\u00e1 nyn\u00ed vede Pandemick\u00fd \u00fa\u0159ad B\u00edl\u00e9ho domu a Rachel Idowuov\u00e1, kter\u00e1 m\u00e1 vazby na H5N1, p\u0159eb\u00edr\u00e1 funkci vedouc\u00ed Jednotky pro reakci na biologick\u00e1 rizika v Rad\u011b n\u00e1rodn\u00ed bezpe\u010dnosti (NSC).<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci nov\u00e9 studie uv\u00e1d\u011bj\u00ed, \u017ee svou vakc\u00ednu testovali infikov\u00e1n\u00edm my\u0161\u00ed druhou chim\u00e9rou: geneticky modifikovan\u00fdm virem ch\u0159ipky H5N1\/PR8, kter\u00fd se skl\u00e1dal z \u010d\u00e1st\u00ed dvou r\u016fzn\u00fdch vir\u016f ch\u0159ipky.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdsledky byly publikov\u00e1ny minul\u00fd m\u011bs\u00edc v \u010dasopise\u00a0<\/span><em><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42429648\/\" target=\"_blank\" rel=\"noreferrer noopener\"><span dir=\"auto\">mSphere<\/span><\/a><\/em><span dir=\"auto\">\u00a0Americk\u00e9 spole\u010dnosti pro mikrobiologii.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Vakc\u00edna obsahuje rekombinantn\u00ed hemaglutinin (HA) H5 z viru pta\u010d\u00ed ch\u0159ipky H5N1 A\/Vietnam\/1203\/2004, ale tento protein nen\u00ed pouze nemodifikovanou kopi\u00ed nativn\u00edho proteinu H5 viru.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci uv\u00e1d\u011bj\u00ed, \u017ee nahradili nativn\u00ed transmembr\u00e1nov\u00fd segment heterologn\u00edm fibritinov\u00fdm z\u00e1hybem, co\u017e vedlo k proteinu H5, kter\u00fd obsahuje slo\u017eku z jin\u00e9ho biologick\u00e9ho zdroje.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Imunostimula\u010dn\u00ed l\u00e1tka obsa\u017een\u00e1 ve vakc\u00edn\u011b je je\u0161t\u011b neobvyklej\u0161\u00ed.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Jmenuje se BECC470s a je syntetickou kopi\u00ed geneticky modifikovan\u00e9 formy lipidu A, biologicky aktivn\u00ed lipidov\u00e9 slo\u017eky bakteri\u00e1ln\u00edho endotoxinu.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdvoj molekuly p\u0159\u00edmo souvis\u00ed s geneticky modifikovanou\u00a0<\/span><em><span dir=\"auto\">bakteri\u00ed Yersinia pestis<\/span><\/em><span dir=\"auto\">\u00a0: V\u011bdci nejprve vytvo\u0159ili lipidovou A strukturu BECC470 s pou\u017eit\u00edm modifikovan\u00e9ho kmene bakterie \u0161k\u016fdce a pot\u00e9 chemicky syntetizovali BECC470 jako purifikovanou verzi t\u00e9to struktury.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u011bdci pot\u00e9 vystavili o\u010dkovan\u00e9 my\u0161i chim\u00e9rick\u00e9mu viru ch\u0159ipky, kter\u00fd \u00fadajn\u011b obsahuje dva segmenty genomu viru pta\u010d\u00ed ch\u0159ipky H5N1 a \u0161est segment\u016f jin\u00e9ho viru ch\u0159ipky, PR8.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">To v\u0161e se d\u011bje v r\u00e1mci p\u0159\u00edpravy l\u00e9ka\u0159sk\u00fdch protiopat\u0159en\u00ed proti \u00fadajn\u00e9mu viru H5N1, kter\u00fd v\u011bdci klasifikuj\u00ed jako pandemickou hrozbu.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">A \u00fa\u010det plat\u00ed ameri\u010dt\u00ed da\u0148ov\u00ed poplatn\u00edci.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Publikace zmi\u0148uje financov\u00e1n\u00ed z kontraktu na adjuvantn\u00ed v\u00fdvoj mezi HHS\/NIH\/NIAID a tak\u00e9 z \u0159ady dal\u0161\u00edch feder\u00e1ln\u00edch program\u016f.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Chcete-li vyj\u00e1d\u0159it sv\u016fj nesouhlas s vakc\u00ednami financovan\u00fdmi z dan\u00ed poplatn\u00edk\u016f, kter\u00e9 obsahuj\u00ed slo\u017eky patogen\u016f zp\u016fsobuj\u00edc\u00edch onemocn\u011bn\u00ed, a tak\u00e9 s v\u00fdzkumem pandemick\u00fdch patogen\u016f, jako je ch\u0159ipka, m\u016f\u017eete kontaktovat\u00a0<\/span><a href=\"https:\/\/www.niaid.nih.gov\/global\/contact-us\" target=\"_blank\" rel=\"noreferrer noopener\"><span dir=\"auto\">NIAID zde<\/span><\/a><span dir=\"auto\">\u00a0,\u00a0<\/span><a href=\"https:\/\/www.nih.gov\/about-nih\/contact-us\" target=\"_blank\" rel=\"noreferrer noopener\"><span dir=\"auto\">NIH zde<\/span><\/a><span dir=\"auto\">\u00a0a\u00a0<\/span><a href=\"https:\/\/www.hhs.gov\/about\/contact-us\/index.html\" target=\"_blank\" rel=\"noreferrer noopener\"><span dir=\"auto\">HHS zde<\/span><\/a><span dir=\"auto\">\u00a0\u2013 zejm\u00e9na pot\u00e9, co Kongres, B\u00edl\u00fd d\u016fm, Ministerstvo energetiky, FBI, CIA a N\u011bmeck\u00e1 feder\u00e1ln\u00ed zpravodajsk\u00e1 slu\u017eba (BND) p\u0159ipustily, \u017ee pandemie COVID-19 byla \u201epravd\u011bpodobn\u011b\u201c d\u016fsledkem laboratorn\u00ed nehody zahrnuj\u00edc\u00ed geneticky modifikovan\u00e9 patogeny.<\/span><\/p>\n<figure class=\"wp-block-image aligncenter size-large\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter\" src=\"https:\/\/substackcdn.com\/image\/fetch\/$s_!6h3o!,w_1456,c_limit,f_webp,q_auto:good,fl_progressive:steep\/https%3A%2F%2Fsubstack-post-media.s3.amazonaws.com%2Fpublic%2Fimages%2F757730b8-2bec-4536-928c-eee841cdaa44_654x859.png\" alt=\"\" width=\"646\" height=\"848\" \/><\/figure>\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong><span dir=\"auto\">Vakc\u00edna obsahuje syntetickou kopii slo\u017eky vyvinut\u00e9 v bakteri\u00edch moru.<\/span><\/strong><\/h2>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">BECC470s je adjuvans v experiment\u00e1ln\u00ed vakc\u00edn\u011b, tj. aktivn\u00ed slo\u017eka, kter\u00e1 m\u00e1 aktivovat imunitn\u00ed syst\u00e9m.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Chemicky vzato je BECC470s um\u011ble vyroben\u00e1 molekula lipidu A.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Lipid A je lipidov\u00e1 slo\u017eka lipopolysacharidu (LPS) vn\u011bj\u0161\u00ed membr\u00e1ny gramnegativn\u00edch bakteri\u00ed a je zodpov\u011bdn\u00fd za velkou \u010d\u00e1st biologick\u00e9 aktivity endotoxin\u016f.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">P\u016fvod t\u00e9to konkr\u00e9tn\u00ed molekuly vede p\u0159\u00edmo k\u00a0<\/span><em><span dir=\"auto\">Yersinia pestis<\/span><\/em><span dir=\"auto\">\u00a0.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Studie z roku 2023 popisuj\u00edc\u00ed BECC470 uv\u00e1d\u00ed\u00a0<\/span><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37483830\/\" target=\"_blank\" rel=\"noreferrer noopener\"><span dir=\"auto\">,<\/span><\/a><span dir=\"auto\">\u00a0\u017ee v\u011bdci vyvinuli z\u00e1kladn\u00ed technologii pomoc\u00ed \u201ebakteri\u00e1ln\u00ed enzymatick\u00e9 kombinatorick\u00e9 chemie\u201c (BECC).<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Podle v\u00fdzkumn\u00edk\u016f byl BECC pou\u017eit k vytvo\u0159en\u00ed nov\u00fdch struktur lipidu A v \u201eatenuovan\u00e9m izol\u00e1tu Yersinia pestis (Yp)\u201c.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci rozli\u0161uj\u00ed dv\u011b verze v\u00fdsledn\u00e9 molekuly BECC470:<\/span><\/p>\n<ul>\n<li><strong><span dir=\"auto\">BECC470b<\/span><\/strong><span dir=\"auto\">\u00a0\u2013 biologick\u00e1 verze odvozen\u00e1 z geneticky modifikovan\u00e9 bakterie moru.<\/span><\/li>\n<li><strong><span dir=\"auto\">BECC470s<\/span><\/strong><span dir=\"auto\">\u00a0\u2013 syntetick\u00e1 verze, kter\u00e1 byla n\u00e1sledn\u011b chemicky vyrobena.<\/span><\/li>\n<\/ul>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Metody nenech\u00e1vaj\u00ed mnoho pochyb o biologick\u00e9m p\u016fvodu biologick\u00e9 verze.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci kultivovali odpov\u00eddaj\u00edc\u00ed kmen\u00a0<\/span><em><span dir=\"auto\">Y. pestis<\/span><\/em><span dir=\"auto\">\u00a0KIM6+, shrom\u00e1\u017edili bakteri\u00e1ln\u00ed bu\u0148ky, extrahovali jejich lipooligosacharid a pot\u00e9 odd\u011blili lipidovou slo\u017eku A.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Sami auto\u0159i popisuj\u00ed BECC470b jako gen poch\u00e1zej\u00edc\u00ed z jeho biologick\u00e9ho\u00a0zdroje\u00a0<\/span><em><span dir=\"auto\">Y. pestis .<\/span><\/em><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u011bdci pot\u00e9 chemicky replikovali tuto geneticky modifikovanou strukturu lipidu A jako BECC470.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Publikace uv\u00e1d\u00ed, \u017ee syntetick\u00e1 verze byla vyrobena za pou\u017eit\u00ed patentovan\u00fdch chemick\u00fdch proces\u016f a vykazovala \u010distotu p\u0159es 99 %.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u0158et\u011bzec fyzick\u00e9ho v\u00fdvoje je tedy jasn\u00fd:<\/span><\/p>\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p class=\"wp-block-paragraph\"><strong><em><span dir=\"auto\">Yersinia pestis<\/span><\/em><span dir=\"auto\">\u00a0, bakterie moru<\/span><\/strong><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u2193<\/span><\/p>\n<p class=\"wp-block-paragraph\"><strong><span dir=\"auto\">geneticky modifikovan\u00fd\u00a0lipid A\u00a0<\/span><em><span dir=\"auto\">Y. pestis<\/span><\/em><\/strong><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u2193<\/span><\/p>\n<p class=\"wp-block-paragraph\"><strong><span dir=\"auto\">biologicky produkovan\u00fd BECC470b<\/span><\/strong><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u2193<\/span><\/p>\n<p class=\"wp-block-paragraph\"><strong><span dir=\"auto\">syntetick\u00e1 kopie, BECC470s<\/span><\/strong><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u2193<\/span><\/p>\n<p class=\"wp-block-paragraph\"><strong><span dir=\"auto\">Vakc\u00edna proti pta\u010d\u00ed ch\u0159ipce H5N1<\/span><\/strong><\/p>\n<\/blockquote>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Vakc\u00edna neobsahuje cel\u00e9 bakterie\u00a0<\/span><em><span dir=\"auto\">Y. pestis<\/span><\/em><span dir=\"auto\">\u00a0.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">BECC470 obsa\u017een\u00e9 ve vakc\u00edn\u011b jsou chemicky vyrobenou verz\u00ed geneticky modifikovan\u00e9 molekuly lipidu A, nikoli materi\u00e1lem, kter\u00fd byl fyzicky extrahov\u00e1n ze sou\u010dasn\u00e9 \u0161ar\u017ee morov\u00fdch bakteri\u00ed.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Replikovan\u00e1 molekula v\u0161ak byla vyvinuta jako geneticky modifikovan\u00e1 slo\u017eka\u00a0<\/span><em><span dir=\"auto\">Y. pestis<\/span><\/em><span dir=\"auto\">\u00a0.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">A je z\u00e1m\u011brn\u011b p\u0159id\u00e1v\u00e1n do vakc\u00edny, aby v t\u011ble vyvolal reakci.<\/span><\/p>\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong><span dir=\"auto\">BECC470s si zachov\u00e1v\u00e1 svou proz\u00e1n\u011btlivou aktivitu b\u011bhem<\/span><\/strong><\/h2>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u011bdci uv\u00e1d\u011bj\u00ed, \u017ee zm\u011bnili bakteri\u00e1ln\u00ed lipid pr\u00e1v\u011b proto, \u017ee lipid A m\u016f\u017ee zp\u016fsobovat ne\u017e\u00e1douc\u00ed \u00fa\u010dinky spojen\u00e9 s endotoxiny.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">BECC470s je hexaacylovan\u00e1, monofosforylovan\u00e1 lipidov\u00e1 struktura A.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Proto mu chyb\u00ed 4&#8242;-fosf\u00e1t, kter\u00fd je p\u0159\u00edtomen v p\u0159\u00edbuzn\u00e9 bisfosforylovan\u00e9 molekule BECC438s.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci vysv\u011btluj\u00ed, \u017ee fosf\u00e1tov\u00e9 skupiny jsou d\u016fle\u017eit\u00e9 pro endotoxinovou aktivitu molekul lipidu A.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">BECC470s v\u0161ak nebyl navr\u017een tak, aby zcela eliminoval biologickou aktivitu lipidu A.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u011bdci cht\u011bj\u00ed, aby to i nad\u00e1le stimulovalo imunitn\u00ed syst\u00e9m.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Specificky testovali BECC470 a p\u0159\u00edbuzn\u00e9 molekuly na jejich schopnost aktivovat TLR4 a NF-\u03baB a tento experiment popsali jako m\u011b\u0159en\u00ed jejich schopnosti \u201epotenci\u00e1ln\u011b spustit z\u00e1n\u011bt\u201c.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">BECC470 aktivovaly tuto sign\u00e1ln\u00ed dr\u00e1hu.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci zjistili, \u017ee aktivace NF-\u03baB byla ni\u017e\u0161\u00ed ne\u017e u biologicky produkovan\u00e9ho BECC470, ale p\u0159esto dosp\u011bli k z\u00e1v\u011bru, \u017ee BECC470 nad\u00e1le funguj\u00ed jako agonist\u00e9 TLR4.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">C\u00edlem v\u00fdvoje genetick\u00e9ho in\u017een\u00fdrstv\u00ed je sn\u00ed\u017eit ne\u017e\u00e1douc\u00ed toxicitu spojenou s bakteri\u00e1ln\u00edm lipidem A a z\u00e1rove\u0148 zachovat dostate\u010dnou proz\u00e1n\u011btlivou a imunostimula\u010dn\u00ed aktivitu, aby byl pou\u017eiteln\u00fd pro vakc\u00edny.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci tvrd\u00ed, \u017ee BECC470s nebyl toxick\u00fd v modelu akutn\u00ed toxicity s jednor\u00e1zovou d\u00e1vkou, kter\u00fd provedla spole\u010dnost Labcorp na kr\u00e1l\u00edc\u00edch.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Publikovan\u00fd \u010dl\u00e1nek v\u0161ak neposkytuje prakticky \u017e\u00e1dn\u00e9 podrobnosti o experimentu.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">To zahrnuje testovanou d\u00e1vku, po\u010det zv\u00ed\u0159at, cestu pod\u00e1n\u00ed, dobu pozorov\u00e1n\u00ed nebo toxikologick\u00e9 koncov\u00e9 body.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Proto nen\u00ed mo\u017en\u00e9 nez\u00e1visle posoudit d\u016fsledky tohoto zji\u0161t\u011bn\u00ed pouze na z\u00e1klad\u011b publikace.<\/span><\/p>\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong><span dir=\"auto\">Protein vakc\u00edny proti pta\u010d\u00ed ch\u0159ipce je chim\u00e9rick\u00fd.<\/span><\/strong><\/h2>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Molekul\u00e1rn\u00ed design odvozen\u00fd z moru nen\u00ed jedinou geneticky modifikovanou slo\u017ekou vakc\u00edny.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Protein H5 viru pta\u010d\u00ed ch\u0159ipky pou\u017e\u00edvan\u00fd jako antigen vakc\u00edny je s\u00e1m o sob\u011b geneticky modifikovan\u00fd hybridn\u00ed nebo chim\u00e9rick\u00fd protein.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci uv\u00e1d\u011bj\u00ed, \u017ee za\u010dali s hemaglutininem H5 z kmenu A\/Vietnam\/1203\/2004 H5N1.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Nevytvo\u0159ili v\u0161ak pouze nezm\u011bn\u011bnou kopii tohoto virov\u00e9ho proteinu.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Podle studie v\u011bdci nahradili nativn\u00ed transmembr\u00e1novou dom\u00e9nu H5 heterologn\u00edm trimeriza\u010dn\u00edm motivem fibritinu foldon.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u201eHeterolog\u201c znamen\u00e1, \u017ee n\u00e1hrada poch\u00e1z\u00ed z jin\u00e9ho biologick\u00e9ho zdroje.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdsledn\u00fd vakcina\u010dn\u00ed antigen m\u00e1 kombinovat protein H5 viru pta\u010d\u00ed ch\u0159ipky s ciz\u00ed fibritinovou slo\u017ekou foldon.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Jednodu\u0161e \u0159e\u010deno, jedn\u00e1 se o chim\u00e9rick\u00fd vakcina\u010dn\u00ed protein H5: \u010d\u00e1st konstruktu se skl\u00e1d\u00e1 z HA viru ch\u0159ipky H5, zat\u00edmco jin\u00e1 \u010d\u00e1st byla nahrazena proteinov\u00fdm prvkem, kter\u00fd nen\u00ed H5.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u011bdci pot\u00e9 zkombinovali tento chim\u00e9rick\u00fd protein H5 s 50 mikrogramy BECC470, synteticky vyroben\u00e9ho adjuvans lipidu A, jeho\u017e design je zalo\u017een na\u00a0<\/span><em><span dir=\"auto\">Y. pestis<\/span><\/em><span dir=\"auto\">\u00a0.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Samotn\u00e1 experiment\u00e1ln\u00ed vakc\u00edna tak kombinuje:<\/span><\/p>\n<p class=\"wp-block-paragraph\"><strong><span dir=\"auto\">chim\u00e9rick\u00fd protein H5 viru pta\u010d\u00ed ch\u0159ipky + syntetick\u00e1 kopie lipidov\u00e9 slo\u017eky geneticky modifikovan\u00e9 bakterie moru.<\/span><\/strong><\/p>\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong><span dir=\"auto\">V\u011bdci k testov\u00e1n\u00ed vakc\u00edny pou\u017eili jinou chim\u00e9ru<\/span><\/strong><\/h2>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci uv\u00e1d\u011bj\u00ed, \u017ee vakc\u00ednu n\u00e1sledn\u011b testovali na druh\u00e9 chim\u00e9\u0159e.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Virus pou\u017eit\u00fd v hlavn\u00edm experimentu na my\u0161\u00edch nebyl nemodifikovan\u00fd virus H5N1.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u00dadajn\u011b se jednalo o geneticky modifikovan\u00fd reassortant ch\u0159ipky v pom\u011bru 6:2, co\u017e znamen\u00e1, \u017ee jeho osm segment\u016f genomu se skl\u00e1dalo ze dvou r\u016fzn\u00fdch vir\u016f ch\u0159ipky.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Dva segmenty (geny k\u00f3duj\u00edc\u00ed H5 a N1) poch\u00e1zely z A\/Vietnam\/1203\/2004 H5N1.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Zb\u00fdvaj\u00edc\u00edch \u0161est segment\u016f genomu poch\u00e1z\u00ed z A\/Puerto Rico\/8\/1934, b\u011b\u017en\u011b ozna\u010dovan\u00e9ho jako PR8.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Jednodu\u0161e \u0159e\u010deno: V\u011bdci vytvo\u0159ili chim\u00e9ru ch\u0159ipky H5N1\/PR8, kter\u00e1 nesla \u00fadajn\u00e9 povrchov\u00e9 slo\u017eky H5 a N1 pta\u010d\u00ed ch\u0159ipky na \u0161estisegmentov\u00e9m vnit\u0159n\u00edm genetick\u00e9m le\u0161en\u00ed jin\u00e9ho ch\u0159ipkov\u00e9ho viru.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u011bdci provedli dal\u0161\u00ed zm\u011bnu u slo\u017eky H5: odstranili jej\u00ed polybazick\u00e9 \u0161t\u011bpn\u00e9 m\u00edsto.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdsledn\u00e1 konfigurace HALo byla navr\u017eena tak, aby byla n\u00edzkopatogenn\u00ed.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Tato studie tedy nedokazuje, \u017ee v\u011bdci zv\u00fd\u0161ili patogenitu viru H5N1.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Sp\u00ed\u0161e to dokazuje, \u017ee v\u011bdci pou\u017eili geneticky modifikovanou, atenuovanou ch\u0159ipkovou chim\u00e9ru k testov\u00e1n\u00ed experiment\u00e1ln\u00ed vakc\u00edny vyroben\u00e9 ze slo\u017eek moru.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Publikace p\u0159ipisuje z\u00e1sluhy v\u00fdzkumnici z Mount Sinai Wein\u011b Sunov\u00e9 za poskytnut\u00ed viru PR8\/H5 pou\u017eit\u00e9ho ve studii.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Experiment se tedy skl\u00e1d\u00e1 ze dvou samostatn\u00fdch \u00fadajn\u00fdch chim\u00e9r:<\/span><\/p>\n<ul>\n<li><strong><span dir=\"auto\">Chim\u00e9ra \u010d. 1<\/span><\/strong><span dir=\"auto\">\u00a0\u2013 antigen vakc\u00edny: protein H5 viru pta\u010d\u00ed ch\u0159ipky obsahuj\u00edc\u00ed ciz\u00ed fibritinovou slo\u017eku foldon.<\/span><\/li>\n<li><strong><span dir=\"auto\">Chim\u00e9ra \u010d. 2<\/span><\/strong><span dir=\"auto\">\u00a0\u2013 testovac\u00ed virus: virus ch\u0159ipky H5N1\/PR8 slo\u017een\u00fd ze segment\u016f genomu dvou r\u016fzn\u00fdch vir\u016f ch\u0159ipky.<\/span><\/li>\n<\/ul>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Mezi nimi se nach\u00e1z\u00ed BECC470s, syntetick\u00e1 verze geneticky modifikovan\u00e9 molekuly lipidu A, kterou p\u016fvodn\u011b produkovala bakterie moru.<\/span><\/p>\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong><span dir=\"auto\">HHS financuje v\u00fdzkum<\/span><\/strong><\/h2>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Ameri\u010dt\u00ed da\u0148ov\u00ed poplatn\u00edci financovali v\u00fdzkum prost\u0159ednictv\u00edm n\u011bkolika feder\u00e1ln\u00edch program\u016f.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Studie zmi\u0148uje podporu ze smlouvy NIH\/NIAID na v\u00fdvoj adjuvantn\u00ed l\u00e9\u010dby:<\/span><\/p>\n<p class=\"wp-block-paragraph\"><em><span dir=\"auto\">HHS-NIH-NIAID-BAA2017 \/ HHSN272201800043C<\/span><\/em><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkum tak\u00e9 z\u00edskal podporu od NIAID-CRIPT, Centers of Excellence for Influenza Research and Response (CEIRR), NIAID-CIVICS a finan\u010dn\u00edch prost\u0159edk\u016f NIH na financov\u00e1n\u00ed provozu region\u00e1ln\u00ed laborato\u0159e pro biologickou ochranu na University of Pittsburgh.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Hlavn\u00ed autor, Devon Riley, tak\u00e9 z\u00edskal finan\u010dn\u00ed prost\u0159edky prost\u0159ednictv\u00edm programu dlouhodob\u00e9ho vzd\u011bl\u00e1v\u00e1n\u00ed a vzd\u011bl\u00e1v\u00e1n\u00ed americk\u00e9 arm\u00e1dy.<\/span><\/p>\n<figure class=\"wp-block-image aligncenter size-large\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter\" src=\"https:\/\/substackcdn.com\/image\/fetch\/$s_!OBQA!,w_1456,c_limit,f_webp,q_auto:good,fl_progressive:steep\/https%3A%2F%2Fsubstack-post-media.s3.amazonaws.com%2Fpublic%2Fimages%2F38f579f2-d040-400d-bf9e-6a4f00762118_1605x555.png\" alt=\"\" width=\"830\" height=\"287\" \/><\/figure>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">S\u00ed\u0165 z\u00fa\u010dastn\u011bn\u00fdch instituc\u00ed zahrnovala Marylandskou univerzitu, Pittsburskou univerzitu a L\u00e9ka\u0159skou fakultu Icahna na Mount Sinai.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkumn\u00edci explicitn\u011b zasazuj\u00ed svou pr\u00e1ci do kontextu hrozby, kterou p\u0159edstavuje potenci\u00e1ln\u00ed pandemie pta\u010d\u00ed ch\u0159ipky.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Jejich experiment\u00e1ln\u00ed vakc\u00edna je proto sou\u010d\u00e1st\u00ed feder\u00e1ln\u011b financovan\u00e9 iniciativy na v\u00fdvoj l\u00e9ka\u0159sk\u00fdch protiopat\u0159en\u00ed, kter\u00e1 by mohla b\u00fdt pou\u017eita v budouc\u00ed pandemii H5N1.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Samotn\u00e1 studie nedokazuje, \u017ee by HHS zvolilo tuto konkr\u00e9tn\u00ed experiment\u00e1ln\u00ed formulaci pro ve\u0159ejn\u00e9 pou\u017eit\u00ed, ani \u017ee by se s n\u00ed Ameri\u010dan\u00e9 museli nechat o\u010dkovat.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Dokazuje to n\u011bco omezen\u011bj\u0161\u00edho, ale konkr\u00e9tn\u00edho: HHS vynakl\u00e1d\u00e1 pen\u00edze da\u0148ov\u00fdch poplatn\u00edk\u016f na v\u00fdvoj a hodnocen\u00ed t\u00e9to technologie jako potenci\u00e1ln\u00ed vakc\u00edny proti H5N1.<\/span><\/p>\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong><span dir=\"auto\">Obchodn\u00ed du\u0161evn\u00ed vlastnictv\u00ed<\/span><\/strong><\/h2>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">V\u00fdzkum financovan\u00fd z dan\u00ed je tak\u00e9 spojen se soukrom\u011b licencovan\u00fdm du\u0161evn\u00edm vlastnictv\u00edm.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Vedouc\u00ed autor Robert K. Ernst ozn\u00e1mil, \u017ee je zakladatelem a v\u011bdeck\u00fdm poradcem spole\u010dnosti TollereBio Corporation.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Publikace v \u201emSphere\u201c uv\u00e1d\u00ed, \u017ee spole\u010dnost TollereBio z\u00edskala licenci na du\u0161evn\u00ed vlastnictv\u00ed od University of Maryland-Baltimore v souvislosti s \u00fadaji prezentovan\u00fdmi ve studii.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Spole\u010dnost TollereBio nen\u00ed v publikaci zm\u00edn\u011bna jako v\u00fdrobce hotov\u00e9 vakc\u00edny proti H5N1.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Je v\u0161ak t\u0159eba poznamenat, \u017ee du\u0161evn\u00ed vlastnictv\u00ed souvisej\u00edc\u00ed s v\u00fdzkumem financovan\u00fdm z feder\u00e1ln\u00edch zdroj\u016f ji\u017e bylo licencov\u00e1no soukrom\u00e9 spole\u010dnosti spojen\u00e9 s jedn\u00edm z hlavn\u00edch v\u00fdzkumn\u00edk\u016f studie.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">\u0158et\u011bzec se tak roz\u0161i\u0159uje i za hranice laborato\u0159e:<\/span><\/p>\n<p class=\"wp-block-paragraph\"><strong><em><span dir=\"auto\">V\u00fdzkum pandemick\u00fdch vakc\u00edn financovan\u00fd z pen\u011bz da\u0148ov\u00fdch poplatn\u00edk\u016f \u2192 du\u0161evn\u00ed vlastnictv\u00ed univerzity \u2192 soukrom\u00e1 komer\u010dn\u00ed licence.<\/span><\/em><\/strong><\/p>\n<h2 class=\"wp-block-heading has-medium-font-size\"><strong><span dir=\"auto\">Z\u00e1v\u011br<\/span><\/strong><\/h2>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Ameri\u010dt\u00ed da\u0148ov\u00ed poplatn\u00edci financuj\u00ed v\u00fdzkum zam\u011b\u0159en\u00fd na p\u0159\u00edpravu na pandemii H5N1, kter\u00e1 zahrnuje pou\u017eit\u00ed dvou chim\u00e9r, geneticky modifikovan\u00e9ho deriv\u00e1tu morov\u00e9 bakterie, z\u00e1n\u011btliv\u00e9 reakce prost\u0159ednictv\u00edm aktivace TLR4 a soukrom\u011b licencovan\u00e9ho komer\u010dn\u00edho du\u0161evn\u00edho vlastnictv\u00ed.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">Vakc\u00edna kombinuje chim\u00e9rick\u00fd antigen pta\u010d\u00ed ch\u0159ipky s BECC470 \u2013 syntetickou verz\u00ed lipidu A p\u016fvodn\u011b odvozen\u00e9ho z Yersinia pestis \u2013 a v\u011bdci uv\u00e1d\u011bj\u00ed, \u017ee ji testovali na samostatn\u00e9 chim\u00e9\u0159e ch\u0159ipky H5N1\/PR8.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">P\u0159\u00edpravek BECC470s byl speci\u00e1ln\u011b navr\u017een k udr\u017een\u00ed proz\u00e1n\u011btliv\u00e9 imunitn\u00ed stimulace a z\u00e1rove\u0148 ke sn\u00ed\u017een\u00ed toxicity lipidu A; publikovan\u00e9 tvrzen\u00ed t\u00fdkaj\u00edc\u00ed se akutn\u00ed toxicity je v\u0161ak zalo\u017eeno na testu s jednor\u00e1zovou d\u00e1vkou na kr\u00e1l\u00edc\u00edch, pro kter\u00fd nejsou uvedeny prakticky \u017e\u00e1dn\u00e9 metodologick\u00e9 podrobnosti.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><span dir=\"auto\">To v\u0161e je vyv\u00edjeno s finan\u010dn\u00ed podporou od HHS\/NIH\/NIAID pro budouc\u00ed kontrolu pandemie, zat\u00edmco souvisej\u00edc\u00ed du\u0161evn\u00ed vlastnictv\u00ed univerzity ji\u017e bylo licencov\u00e1no soukrom\u00e9 spole\u010dnosti spojen\u00e9 s hlavn\u00edm autorem studie.<\/span><\/p>\n<p class=\"wp-block-paragraph\"><strong><em><span dir=\"auto\">Od Jona Fleetwooda<\/span><\/em><\/strong><\/p>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-8c57238 quelle dc-has-condition dc-condition-empty elementor-widget elementor-widget-heading\" data-id=\"8c57238\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n<div class=\"elementor-widget-container\">\n<h3 class=\"elementor-heading-title elementor-size-default\"><span style=\"font-size: 10pt;\"><a href=\"https:\/\/jonfleetwood.substack.com\/p\/hhs-funds-chimeric-bird-flu-pandemic\" target=\"_blank\" rel=\"nofollow noopener\"><span dir=\"auto\">Zdroj<\/span><\/a><\/span><\/h3>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Ameri\u010dan\u00e9 za to plat\u00ed a mohli by b\u00fdt po\u017e\u00e1d\u00e1ni, aby to p\u0159evzali. Ministerstvo zdravotnictv\u00ed a&#8230;<\/p>\n","protected":false},"author":2,"featured_media":113535,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[7],"tags":[1361,1975,259,37,579],"_links":{"self":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/posts\/113534"}],"collection":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/comments?post=113534"}],"version-history":[{"count":0,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/posts\/113534\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/media\/113535"}],"wp:attachment":[{"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/media?parent=113534"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/categories?post=113534"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.infokuryr.cz\/n\/wp-json\/wp\/v2\/tags?post=113534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}